Intra-abdominal adhesions can be a considerable source of morbidity and mortality. A Simple Mouse Model for the Study of Human Immunodeficiency Virus Intraperitoneal (IP) Technique The dose is 3 ml/4.5 kg (10 lbs) of euthanasia solution. Thus, IP and AI routes are free of pulmonary embolism issue. If there is negative pressure, proceed with the injection depress the plunger until the solution has been fully administered. injection, or EBI) remains the most humane method available. The dynamic in vivo distribution of bone marrow-derived mesenchymal stem cells after infusion. IHC was performed according to the standard procedures. 4D,E). Ng, S. C. et al. IBD can be painful and persistent for life, which might decrease the quality of life. Not only in animal studies, some clinical trials using MSCs to treat osteogensis imperfect (OI) in GvHD, also showed less than 1% cell were detected in target organ18,19,20. All fluorescent imaging procedures were conducted on small animal imaging system (IVIS Kinetics; Caliper Life Science). Yui, S. et al. Intraperitoneal (IP) route of drug administration in laboratory animals is a common practice in many in vivo studies of disease models. Gastroenterology 145, 158165 e152, doi: 10.1053/j.gastro.2013.04.007 (2013). Garcia-Bosch, O., Ricart, E. & Panes, J. Meanwhile, GFP+ MSCs (collected from transgenic mice) were used to determine MSCs specific location. Atreya, R. & Neurath, M. F. Chemokines in inflammatory bowel diseases. Stem Cell Res Ther 3, 51, doi: 10.1186/scrt142 (2012). Comparison of molecular profiles of human mesenchymal stem cells derived from bone marrow, umbilical cord blood, placenta and adipose tissue. Normal mouse IgG was used as a substitute for the primary antibody as negative control. 1B). Li, Y. P. et al. Caplan, A. I. There are few accounts of the use of intraperitoneal injections prior to 1970. Your peritoneal (payr-ih-toh-NEE-ul) space is the area between the muscles and organs in your abdomen (belly). Intraperitoneal Route of Drug Administration: Should it Be Used in Drug Deliv. The authors give their special thanks to the Dr. Yingchun Jia and Zhen Chen for their help in histological evaluation. Department of Digestive Diseases, State Key Laboratory of Cancer Biology, Xijing Hospital, Fourth Military Medical University, Xian, 710032, China, Min Wang,Cong Liang,Hao Hu,Lin Zhou,Bing Xu,Xin Wang,Ying Han,Yongzhan Nie,Jie Liang&Kaichun Wu, Department of Gastroenterology, Xian Childrens Hospital, 710006, China, Department of Respiratory and Gastroenterology, Second Peoples Hospital, Xian, 710005, China, Department of Gastroenterology, PLA No.5 Hospital, Yinchuan, 750004, China, You can also search for this author in and JavaScript. From whole-body disease recovery, the amount of MSCs colons engraftment, tissue histological evaluation as well as levels of cytokines and TSG-6. Injection Techniques, Restraint, & Handling for Mice and Rats Google Scholar. Google Scholar. [citation needed], In humans, the method is widely used to administer chemotherapy drugs to treat some cancers, particularly ovarian cancer. Available at: http://www.cdc.gov/ibd/ (Accessed: 7th April 2016) (2014). Colitis showed varying degrees of alleviation after three ways of MSCs transplantation and the IP injection showed the highest survival rate of 87.5% and displayed the less weight loss and quick weight gain. It can significant reduce failure puncture and associated organ damages caused by blind needle puncturing. Intraperitoneal Injection in the Mouse - Research Animal Training By systemic evaluation of epithelium and lymphocyte infiltration, the IP injection group showed the relatively lower severity score compared with AI group (P=0.034) and IV (P=0.002), respectively. ISSN 2045-2322 (online). Afterwards, the samples were incubated with Ki-67 (1:200; Santa Cruz) or FOXP3 (1:100; Antibodies-Online, Aachen, Germany) overnight at 4C. Provided by the Springer Nature SharedIt content-sharing initiative. and H.H. Stem Cells 29, 15721579, doi: 10.1002/stem.708 (2011). Inflammatory Scores were calculated using a score system66: Epithelium (E): 0, normal morphology; 1, loss of goblet cells; 2, loss of goblet cells in large areas; 3, loss of crypts; 4, loss of crypts in large areas. Li, C. Y. et al. Furthermore, the corresponding mesentery lymph nodes (MLNs) also showed relatively high MSCs existence (Fig. 4C). Inactivating IL34 promotes regenerating muscle stem cell expansion and 7 . PDF Chris Quigley Essentials Full Spectrum Curriculum (2023) Injection of substances into peritoneum (body cavity), Current Drugs Delivered Intraperitoneally, "Why i.p. PubMed Central Gastrointestinal Absorption of Insulin-like Growth Factor Study showed that genetically modified MSCs to increase CXCR-4 expression will lead to an increase of MSCs migration to intestinal with radiation enteritis and then resulted in disease improvement24. Tuning the Physicochemical Characteristics of Particle-Based Carriers for Intraperitoneal Local Chemotherapy. DailyMed - PIPERACILLIN AND TAZOBACTAM injection, powder, lyophilized Bookshelf PDF EUTHANASIA REFERENCE MANUAL - HumanePro doi: 10.1002/14651858.CD005284.pub3. Figure 1. (B) MSCs signal quantification of five main organs (lung, liver, spleen, kidney and large bowel) and corresponding MLNs. PubMed & Yong, K. Factors that influence short-term homing of human bone marrow-derived mesenchymal stem cells in a xenogeneic animal model. Goncalves Fda, C. et al. Infiltration (I): 0, no infiltrate; 1, infiltrate around crypt basis; 2, infiltrate reaching to lamina muscularis mucosa layer; 3, extensive infiltration reaching the muscularis mucosa with abundant edema; 4, infiltration of the submucosa layer. The fluorescence imaging disclosed higher intensity of engrafted cells in inflamed colon and the corresponding mesentery lymph nodes (MLNs) in IP and AI groups than the IV group. Shariati M, Zhang H, Van de Sande L, Descamps B, Vanhove C, Willaert W, Ceelen W, De Smedt SC, Remaut K. Pharm Res. (CS), Intraperitoneal, Polymicrobial, and Antibiotics available under aCC-BY 4.0 International license. gynecological and gastrointestinal cancers and has shown very promising results. [2], There has been some debate on whether intraperitoneal injections are the best route of administration for experimental animal studies. Cosnes, J., Gower-Rousseau, C., Seksik, P. & Cortot, A. Other gap junction blockers were also occasionally reported to . [3] The study however did not find an increase in conception rate when compared to mating. Zhang, B. et al. Half of the volume of your D-luciferin substrate can be injected on one side of the animal and the other half of the volume on the opposite side. The need of intestinal resection in CD has remained stable8 and the colectomy rates in UC were still for 20% and 30% within 10 and 25 years of diseases duration9. 3) and the IV group preserved fewer cell number. ***P<0.001; **P<0.01. 2A). The authors declare no competing financial interests. Pentobarbital sodium Injection Caution. Google Scholar. Please enable it to take advantage of the complete set of features! analyzed the data, J.L. Regenerative medicine 1, 589604, doi: 10.2217/17460751.1.4.589 (2006). In 1996, intraperitoneal (IP) administration of cisplatin plus intravenous (IV) cyclophosphamide proved superior to both drugs given intravenously at the same doses-which, at the time, was the standard treatment in the United States. Best way for mesenchymal stem cells transplantation for colitis. PubMed Endoscopic fluorescence imaging experiments were performed at 1-h postinjection of FA-NPs, celecoxib/FA-NPs or FNC-NPs using a Karl Storz-Endoskope System. Evid Based Complement Alternat Med. Ahmadian Kia, N. et al. The MSCs may exert their therapeutic effects on mainly three following aspects: firstly, to differentiate and replace the damage tissue; secondly, to secrete soluble immunomodulatory bioactive molecules and establish a regenerative milieu; thirdly, cell-to-cell contact and interaction with immune cells. One study attempted to diagnose hepatic hydrothorax with the use of injecting Sonazoid intraperitoneally. Twenty-five Kunming mice were exposed to Fe 3 O 4-nanoparticles via intraperitoneal injection daily for 1 week at doses of 0, 5, 10, 20, and 40 mg/kg. 6A). Depletion of O6-alkylguanine-DNA alkyltransferase activity in mammalian Evid Based Complement Alternat Med. It is more often applied to animals than to humans. PubMed Central At 24h after injection, the intensity of free dye was 100 folds less than the DiR labeled cells (from the bar value. Dig Dis 28, 386394, doi: 10.1159/000320392 (2010). Intraperitoneal injection carries the risk of causing internal injury or of misplaced injection into a blood vessel or an organ, particularly the large thin-walled cecum or urinary bladder. The Journal of clinical investigation 117, 258269, doi: 10.1172/jci29159 (2007). MSCs have been well characterized regarding their ability to produce a range of cell cytokines, which have a profound effect on modulating the immune system. Kyriakou, C., Rabin, N., Pizzey, A., Nathwani, A. Ceelen W, Braet H, van Ramshorst G, Willaert W, Remaut K. Expert Opin Drug Deliv. Kenny et al. The .gov means its official. In IP group, the MSCs existence can be detected at day 1, but gradually decreased with the time, GFP+ cells can be hardly identified in inflamed colon after day 3 (data not shown). The coefficient 13.5 was empirically determined such that 1U MPO activity represents the amount of enzyme that will reduce 1 mol peroxide/min. In a previous study, 14 mice were exposed to SWCNTs through intravenous (IV) injection to determine whether there were any adverse effects caused by SWCNTs on the mouse brain and also to find a safe application dosage if possible. For some severe cases, IBD may lead to the life-threaten complications5. However, human insulin . The Scheme (Fig. Human CD3 + T cells were weakly detected during the first 2 to 3 weeks after intraperitoneal injection of human PBMCs; however, 4 weeks after intraperitoneal injection, the percentage of human CD3 + T cells rapidly increased, reaching peak levels of 79.1%. Lee et al. Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing. A consensus report written on behalf of the European League Against Rheumatism (EULAR) and the European Group for Blood and Marrow Transplantation (EBMT). Federal law restricts this drug to use by or on the order of a licensed veterinarian. Nonmyeloablative stem cell therapy enhances microcirculation and tissue regeneration in murine inflammatory bowel disease. Frontiers | Efficacy of intratympanic or postauricular subperiosteal J Vis Exp, doi: 10.3791/1488 (2010). Article Furthermore, in previous low-incidents Asian area, the incidence and prevalence are also reported to increase in recent decades3,4. Increased initial cell dose ensures an increased number of cells that reach to the injury site. -Lactalbumin is an example of a "retired" self-protein because its expression in normal . We also evaluated serum TSG-6 in three MSCs administration route. At day 5, mice were randomized and injected with MSCs (1106, 200L in volume) by three delivery routes (IP, AI and IV). The study overall defines appropriate doses and toxicity levels of dl1520 when injected intraperitoneally. Firstly, peritoneal cavity provides an isolated, hemodynamically stable, sterile and nutritious environment with enough growth spaces, which are very critical factors for aggregates forming. Google Scholar. Integrative genetic analysis identifies FLVCR1 as a plasma-membrane . This study goes over the dosages, adverse effects, and more of using intraperitoneal injections of anesthesia.[18]. Gynecol Oncol 81, 7781, doi: 10.1006/gyno.2000.6108 (2001). In humans respiratory side-effects were reported in five subjects taking mefloquine, but these cases constituted only 0.1% of studied population (Lobel et al., 2001). Essentials of Human Nutrition - Jun 13 2022 . eCollection 2021. For this reason, more MSCs aggregates would form for IP route and produce higher level of serum TSG-6 under TNF- activation, which help reducing the colon inflammation. Intraperitoneal but not intravenous cryopreserved mesenchymal stromal cells home to the inflamed colon and ameliorate experimental colitis. These findings could be advanced by studying how the route of injection affects the organs in the peritoneal cavity. Injections are given along the midline caudal to the umbilicus or low on the right lateral abdomen. Thus, we have succeeded to produce patient-like tumor colonization of the peritoneal cavity and hepatic metastasis with the human EAC cell line OE19 by intraperitoneal injection in SCID mice. Fresh feces from animals were collected for occult blood test, using fecal occult blood kit (Baso Diagnostics Inc., Zhuhai, China) according to the manufactures recommendations. . 7A). Google Scholar. Cell and tissue research 347, 419427, doi: 10.1007/s00441-011-1306-3 (2012). Highlighting the importance of choline metabolism . Blood 118, 330338, doi: 10.1182/blood-2010-12-327353 (2011). Inflammatory bowel disease (IBD). Google Scholar. Insert the needle at a shallow angle through the . Epidemiology and natural history of inflammatory bowel diseases. Gao, J., Dennis, J. E., Muzic, R. F., Lundberg, M. & Caplan, A. I. It is more often applied to animals than to humans. Intraperitoneal Route of Drug Administration: Should it Be Used in In contrast, the DSS control showed 15.1% Ki-67+ cells/crypt. There was a dose-dependent decrease in AGT activity in liver tissues of CD-1 mice to 24% of basal levels after four hourly intraperitoneal injections of O 6-methylguanine (110 mg/kg). was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity . Intraperitoneal Injection Is Not a Suitable Administration Route for Some of the examples depict situations where IP injections are not ideal, while others prove the advantageous uses if this delivery method. Here, we also compare the expression of FoxP3, which is frequently used to quantitate Treg cells. The IP injection showed longer colon, with mean length of 8.24cm compared with 6.90cm in AI group (P=0.0057) and 6.14cm in IV group (P<0.001; Fig. Though IP injection showed benefits in many human and animal studies, even recommend by National Cancer Institute (NCI) for advanced ovarian chemotherapy, it is more often applied to animals than to humans for the time being57. Intraperitoneal (ml) Footpad (ml) Mouse <0.1 . Intraperitoneal Chemotherapy - Memorial Sloan Kettering Cancer Center official website and that any information you provide is encrypted Cytokine in colonic protein extracts were determined by ELISA Kits according to the manufacturers instructions (R&D Systems, Minneapolis, MN). T1DM was induced by intraperitoneal injection of STZ (Sigma-Aldrich, St. Louis, MO, USA) in 25 mM sodium citrate (pH 4.5), with the dosage of 200 mg/kg body weight, into 10-week-old BALB/c mice with the body weight of 2325 g. . However, most of these studies were focus on its therapeutic effects or mechanisms. PMC Fluorescent intensity was quantified and processed using the Living Image Software (Version 4.2; Caliper Life Science). Truncal blocks and neuraxial . Samples were centrifuged at 30,000g for 20minutes and stored at 80C until cytokine determination. (A) Scheme of the treatment, the day of MSCs injection was defined as day 0. However, if proper placement of the needle occurs, this is not necessary. Cancers | Free Full-Text | Targeted Vaccination against Human 4A,B). There are few examples of the use of intraperitoneal injections in humans cited in literature because it is mainly used to study the effects of drugs in mice. Intraperitoneal (IP) route of drug administration in laboratory animals is a common practice in many in vivo studies of disease models. Intraperitoneal - an overview | ScienceDirect Topics Ko, I. K. et al. L.Z. 2018 Nov;25(1):846-861. doi: 10.1080/10717544.2018.1455764. Blood 122, e23e32, doi: 10.1182/blood-2013-04-495119 (2013). Analysis of tissues following mesenchymal stromal cell therapy in humans indicates limited long-term engraftment and no ectopic tissue formation. FoxP3+ cells were greatly increased in MSCs therapy. This is really an interesting finding, however exact mechanisms are still unknown. The fecal occult blood test on the day 3 also showed nearly complete absence of occult blood in IP group. This shows an important limitation to the use of IP therapy. Although controversial, intraperitoneal use in ovarian cancer has been recommended as a standard of care. Experiments with microspheres has showed that most of particle of this size would be clear out by the lung17. The IP technique is an easy technique to master. Treatment for peritoneal dialysis-associated peritonitis. Article But for the IP injection, because of more cell accumulation in the inflamed colon, less initial cells might reach minimum effective cell dose. Alavi S, Haeri A, Mahlooji I, Dadashzadeh S. Pharm Res. Models should begin with local tissue infection, so that bacterial . Like pharmacological treatment, the cell dosing might also be an important issue for MSCs therapeutic success21. prepared Figure 4. Sirius red staining showed disorganized collagen fibers in DSS control colon, which were diffusely distributed in the injured colon wall. PubMed Central Cell stem cell 9, 1115, doi: 10.1016/j.stem.2011.06.008 (2011). For intraperitoneal administration, restrain the mouse with one hand and tilt the head backward to expose the abdomen. Currently, there are a handful of drugs that are delivered through intraperitoneal injection for chemotherapy. Rep. 6, 30696; doi: 10.1038/srep30696 (2016). One of the earliest recorded uses of IP injections involved the insemination of a guinea-pig in 1957. Our findings showed that the GFP+ MSCs were migrated to the inflamed colon, even passed through the whole intestinal wall and reached the luminal side. of the needle exposed to assure 4-5mm injection depth. 2A). Hsiao, S. T. et al. drug availability and human abuse potential; 10. compatibility with species, age, and health status; 11. Slider with three articles shown per slide. Rodent (large / mouse) oral (intragastric), intravascular administration (tail vein injection, intraperitoneal injection, intramuscular injection and subcutaneous injection) Intragastric administration and oral taking of non-rodent (dog / monkey) solution and intravenous injection of extremities. Role for interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells. Sphingosine-1-phosphate links persistent STAT3 activation, chronic intestinal inflammation and development of colitis-associated cancer. Skin application of non-rodents (miniature pigs) Surg Clin North Am 87, 727741, doi: 10.1016/j.suc.2007.03.004 (2007). PDF Guidelines for the Research Use of Adjuvants - National Institutes of A protocol for isolation and culture of mesenchymal stem cells from mouse compact bone. Acceptable Method (s) Injected barbiturates and barbiturate combinations. Furthermore, the body weight changes displayed the less weight loss and quick weight gain of IP injection group and the maximum group difference among was reached on day 3 (Fig. Would you like email updates of new search results? n=68 mice/group; n=3 for the control and naive (health mice) group. The immunohistochemistry indicated higher repair proliferation (Ki-67) and more FoxP3+ cells accumulation of IP group. In anal injection group, same amount of cells were suspended in 200L diluted Matrigel in PBS (1:20) and the cell suspension was instilled into colonic lumen by a syringe with flexible catheter 4cm in length and 2mm in diameter, according to the previous study64. The https:// ensures that you are connecting to the
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